Reliable guarantee for the accuracy of the temperature. Temperature extended control mode which is closer to required experiment temperature control and is able to effectively avoid the system error caused by the disaccord of the temperature points among the instrument's display temperature, actual block temperature and the temperature required for reagents. So as to improve the accuracy of the experiment and ensure the high efficiency, strict temperature control debugging program makes sure that each instrument can meet the needs of different experiments. 12 channel temperature probes detect simultaneously, which ensure the homogeneity of sample temperature. The hermetic-space technique can efficiently eliminate PCR marginal effect. The technique of outside temperature probe tracing the inside curve testing can effectively ensure the accuracy of sample temperature.